Projects

Our projects

Our lab works with development of computational tools for biological manipulation and integration, as well as assembly of genomic and transcriptomic data (RNA-SEQ), both from NGS platforms, and analysis of gene expression regulatory elements

Analysis of the functional genomics of Corynebacterium pseudotuberculosis ovitis and equi biotypes under different biologically relevant stress conditions

Project certified by the coordinator Artur Luiz da Costa da Silva on 05/14/2013.

Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Coordinator / Vasco - Member / Anne Cybelle Pinto - Member / Wanderson Marques Silva - Member / CARNEIRO, ADRIANA - Member.
Financier (s): National Council for Scientific and Technological Development - Financial aid.

ARTEMan: Transfer of resistance to antibiotics between the environment and man


Project certified by the coordinator Artur Luiz da Costa da Silva on 08/08/2015.

Description: International cooperation project FCT CAPES Announcement CAPES-FCT 039/2014. University of Aveiro Profa. Dr. Isabel da Silva Henriques ..
Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Coordinator / Isabel da Silva Henriques - Member.

Evaluation of the Bioremediation Potential of the Atlantic Amazon by the Petroleum Industry

Project certified by the coordinator Artur Luiz da Costa da Silva on 01/07/2014.

Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Coordinator / Vivian Pellizari - Member / GRAÇAS, DIEGO A - Member / Adriana R Carneiro - Member.

Pangenomic Analysis of Emerging Pathogenic Corinebacteria


Project certified by the coordinator Artur Luiz da Costa da Silva on 01/07/2014.

Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Coordinator / Anne Cybelle Pinto - Member / Barauna - Member / Thiago Lopes - Member / CARNEIRO, ADRIANA - Member / GRAÇAS, DIEGO A - Integrante / FILHO, LUCIANO CF - Member / Pablo Henrique Caracciolo Gomes de Sá - Member / Fernanda Alves Dorella - Member / Adonney Allan de Oliveira Veras - Member.

Study of the Interatoma of Corynebacterium pseudotuberculosis in Mus musculus cell line


Description: Universal 01/2016 - Track B.
Status: In progress; Nature: Research.
Students involved: Undergraduate: (2) / Academic Master: (6) / Doctorate: (6).

Members: Rommel Thiago Juca Ramos - Coordinator / Vasco - Integrante / Pacheco, Luis GC - Member / Flavia Aburjaile - Member / BARAUNA, RA - Member / Artur Luiz Da Costa da Silva - Member / Siomar Castro Soares - Member / Adriana R Carneiro - Integrante / Luís Carlos Guimarães - Member / Adonney Allan de Oliveira Veras - Integrante / kenny C Pinheiro - Member / Yan Pantoja - Member / LOPES DE SOUSA, AILTON - Member / Jefferson Magalhães de Moraes - Member / Amalia Raiana Fonseca Lobato - Member / Gabriel Rosa Santos Muge - Member.
Financier (s): National Council of Scientific and Technological Development - Financial aid.Number of guidelines: 1

The genome of the Marajó buffalo


Project certified by the coordinator Artur Luiz da Costa da Silva on 08/08/2015.

Description: Recent advances in molecular biology have led to rapid obtaining of complete genomes of domestic animals. In relation to genomics applied to cattle raising, gene regions linked to milk and cheese production and quality, the incidence of hereditary diseases, resistance and susceptibility to verminosis, meat tenderness and marbling, weight gain, among others were identified. The trend is an increase in the generation of new information on gene regions and genes that may influence the production, health, and quality of the populations of animals raised for human consumption. This information had a great impact on the design of new technological options for production systems, especially those associated with genetic improvement. Animal breeding programs are based on the correct identification of superior animals, who will be the progenitors of future generations. Reproductive techniques, such as artificial insemination and embryo transfer, have been used by researchers to increase the number of progenies of genetically superior animals. In 2008 the Government of Pará implements its Genome Network consolidating the competence in Genomics and Bioinformatics in the Amazon Region. This governmental action aims to offer biotechnology services that meet the demand of society and the region. Research and teaching activities developed in the Genoma Pará Network allowed the creation of an active nucleus in Genomics and Bioinformatics in Brazil, which currently has 51 microorganism sequencing projects in the NCBI, making this research program the largest Latin American collaborator together to NCBI. Another achievement worth mentioning was the recent publication of the first genome of an Amerindian carried out integrally at UFPA.
Status: In progress; Nature: Research.
Students involved: Undergraduate: (2) / Academic Master: (1) / Doctorate: (1).

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Coordinator / Silvanira - Member / Adriana R Carneiro - Member.
Financier (s): Amazonia Paraense Foundation for Research Support - Financial aid.

Academic cooperation network for the study and development of tools for structural and functional genomics

Project certified by the coordinator Vasco Ariston de Carvalho Azevedo on 08/13/2014.

Description: CAPES Edital nº 51/2013 COMPUTER BIOLOGY.
Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Integrante / Vasco - Coordinator / CARNEIRO, ADRIANA - Member.
Financier (s): Coordination of Improvement of Higher Level Personnel - Financial Aid.

Analysis of the transcriptional profile of Corynebacterium pseudotuberculosis biotype equi under conditions of environmental stresses


Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Integrante / Vasco - Member / Anne Cybelle Pinto - Member / Adriana R Carneiro - Coordinator.

Genome of Brazilian birds, phylogenomics, speciation, conservation and neural gene networks linked to singing (Beija-flor and sabiá) and imitation of speech (Parrots) - Sisbioaves

Status: In progress; Nature: Research.
Students involved: Undergraduate: (2) / Academic Master: (3) / Doctorate: (1).

Members: Rommel Thiago Juca Ramos - Member / Schneider, Maria Paula C. - Coordinator / Maria Sueli Felipe - Member / Claudio Mello - Member / Evonnildo Gonçalves - Member.
Financier (s): National Council for Scientific and Technological Development - Financial Aid / Coordination for the Improvement of Higher Education Personnel - Exchange.

Identification of Proteins Producing the Type VII Secretion System of Corynebacterium pseudotuberculosis under biologically relevant stress conditions and evaluation of their roles in host interaction mechanisms

Project certified by the coordinator Artur Luiz da Costa da Silva on 08/08/2015.

Description: Caseous lymphadenitis is a chronic disease caused by facultative intracellular Gram-positive bacteria of the species Corynebacterium pseudotuberculosis, which affects small ruminants. This bacterium can infect both economically important animals (eg goats, sheep, cattle and horses) as well as wild animals and humans. The pathology is characterized by the presence of granulomas in the visceral and superficial lymph nodes, causing great economic losses due to the condemnation of carcasses, skin and reduced reproductivity. In Brazil, estimates show that most of the herds are infected, a fact that is exacerbated by the lack of satisfactory prophylactic methods for the control of CL. There is still limited knowledge on the molecular determinants of virulence of C. pseudotuberculosis, as well as on the control of its gene expression. It is known that during the infectious process, this bacterium is exposed to a range of different environments, from the point of entry into the host, through the lymphatic system, to intracellular replication within macrophages and the establishment of lesions. Thus, the bacterial response to intracellular stresses represents an important acting factor in this process. Many proteins secreted by pathogens are released or "injected" into host cells to modify cellular physiology and promote colonization with the aid of effector proteins and toxins. Specific secretory machinery of Gram-positive bacteria is the type VII (T7SS) system, which has been extensively described in M. tuberculosis because it consists of three essential proteins for the virulence of this bacterium. There are reports of the presence of this system in corynebacteria, but has not been studied deeply so far. In this context, the objective of this project is to identify and evaluate the T7SS effector proteins (PEs) of C. pseudotuberculosis, taking into account the conditions of the intracellular environment. For this, we will subject the bacteria to stress conditions and identify the differentially expressed proteins. In parallel, mutant lines will be generated for T7SS PEs to evaluate the viability of the macrophages infected with these lines. Finally, we will evaluate the persistence of the disease in mice after infection with the mutant lines, as well as the production of cytokines. We hope that through this project new important PEs for the virulence of C. pseudotuberculosis will be identified under stress conditions, and so we can advance our studies in the search for more efficient methods to control the diseases caused by this pathogen.
Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Coordinator / Basque Ariston de Carvalho Azevedo - Member / SEYFFERT, NUBIA - Member.
Financier (s): CAPES - Anhanguera Center for Promotion and Social Education - Financial aid.

Microbial Life in the Antarctic Cryosphere: Climate Change and Bioprospecting (MICROSPHERE)


Project certified by the coordinator Artur Luiz da Costa da Silva on 01/07/2014.

Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Coordinator / Vivian Pellizari - Member / Adriana R Carneiro - Member. Financier (s): National Council for Scientific and Technological Development - Financial aid.

Network for Academic Cooperation in Functional Genomics and Biology of Microorganism Systems

Project certified by the coordinator Artur Luiz da Costa da Silva on 08/11/2015.

Description: EDITAL N 071/2013 - NATIONAL PROGRAM OF ACADEMIC COOPERATION - PROC ..
Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Coordinator / Vasco - Member / Adriana R Carneiro - Member / Andrea Kelly Ribeiro dos Santos - Member.
Financier (s): Coordination of Improvement of Higher Level Personnel - Financial Aid.

Prospecting of antibiotic resistance and microbial diversity in the surrounding tributaries of the city of Santo Domingo, using techniques of metagenomics and NGS

Description: The objective of this research is to characterize microbial diversity, as well as to identify and characterize resistance and transfer mechanisms of antibiotic resistance between the environment and man, in the rivers that surround the city Santo Domingo in the Dominican Republic. Understanding microbial communities will allow the development of strategies and methodologies to reduce the impact and risks that antibiotic-resistant bacteria can cause. Thus, we will create a database of environmental monitoring showing the characteristics of each of the impacted areas.
Status: In progress; Nature: Research.
Students involved: Undergraduate: (2) / Academic Master: (2) / Doctorate: (3).

Members: Rommel Thiago Juca Ramos - Coordinator / Artur Luis da Costa da Silva - Member / Rafael Baraúna - Member / Omar Paíno Perdomo Sánchez - Member / Luis Enrique Rodríguez - Member / Edian Franklin de Los Santos - Member / Rosanna Carreras - Integrante / Víctor V. Calderón SotoEsperanza E. Mendoza Reyes - Member.
Financier (s): Instituto Tecnológico de Santo Domingo - Cooperation.

Reconstruction and Modeling of Metabolic Pathways in the Cyanobacterial Genome Scale Microcystis aeruginosa SPC777

Description: The project aims to identify microcystis aeruginosa SPC77 metabolic pathways from the complete genome of the microorganism, develop the in silico model of Microcystis aeruginosa SPC77 and use this model to identify products of biotechnology interest as well as improve their productivity.
Status: In progress; Nature: Research.

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Member / Maria Paula Cruz Schneider - Coordinator.

Study of the interatoma and exosome in Corynebacterium pseudotuberculosis for research of new therapeutic targets

Description: Microorganisms of the genus Corynebacterium are classified in the CMNR group, which also includes the genera Mycobacterium, Nocardia and Rhodococcus. It comprises one of the most important groups of the bacterial domain since it contains species of medical, veterinary and biotechnological interest (Khamis et al., 2004; Dorella et al., 2006). Among these species, we can highlight the pathogen of animals Corynebacterium pseudotuberculosis, which is the etiological agent of CLA caseous lymphadenitis, a disease that mainly affects sheep and goats around the world (Dorella et al., 2006). Worldwide, Agribusiness has suffered considerable losses due to the infection caused by the pathogenic bacterium C. pseudotuberculosis in its different hosts. This bacterium was initially identified as a microorganism causing caseous lymphadenitis in goats and sheep, but has also been isolated from other species such as camelids, cattle, buffaloes, horses causing ulcerative lymphangitis and even in humans (Yeruham et al. 2004; Williamson, 2001; Selim, 2001), and there are still no solutions to effectively combat the diseases caused by this microorganism. Brazil became a reference in the molecular analysis of this species, and until June 2013, 15 lines of C. pseudotuberculosis were isolated and their genomes were sequenced and analyzed, revealing important characteristics of the species and adding new knowledge about the economic and biological problems of C. pseudotuberculosis (Soares et al., 2013). The caseous lymphadenitis is characterized by the formation of abscesses containing pus of greenish-yellow coloration and viscous consistency. It can occur under two clinical forms, a superficial one that affects the palpable lymph nodes (external LC), and another visceral form that affects the internal lymph nodes (visceral LC) and organs like lung, kidney, liver (Dorella et al., 2006). Treatment is mainly by the use of antibiotics, but it is expensive and ineffective since the drugs do not penetrate the abscess capsule. That is why it is important to develop a potent, safe and truly protective vaccine. Some vaccines have already been investigated, including DNA, but the result is still not satisfactory (Dorella et al., 2006; Baird & Fontaine, 2007). The diagnosis of caseous lymphadenitis is mainly clinical. It is observed in the affected animal, the formation of large lumps, usually in the head, jaw, and neck. Because it is contagious, the disease spreads in herds mainly through direct contact between animals. In some cases, the infection produces few obvious symptoms, remaining undetectable until a postmortem examination is performed. Thus, late clinical diagnosis is one of the main problems (Alves & Pinheiro, 2000). When the nodular lesion (the lump) is verified, the animal may already be seriously compromised, with loss of weight and yield of meat and/or milk production. In this case, the only solution to avoid propagation is the disposal of affected animals, including the carcass, without the use of leather (Veschi, 2005). The size and severity of the lesion depend on the initial number of microorganisms penetrating the host tissue, the rate of multiplication of the microorganism, and the host's susceptibility to defending itself from the bacterium, however, this appears to have little relation to the duration of infection. Pathogenesis begins when bacteria enter the body, multiply and are phagocytosed. Intracellular survival in phagocytes is the main factor for dissemination and eventual formation of abscesses, which is mediated by virulence factors (Jolly, 1966).
Status: In progress; Nature: Research.
Students involved: Academic Master: (2) Ph.D.: (3).

Members: Rommel Thiago Juca Ramos - Member / Artur Luis da Costa da Silva - Member / Basque Ariston de Carvalho Azevedo - Member / Adriana Ribeiro Carneiro - Coordinator.
Financier (s): Coordination of Improvement of Higher Level Personnel - Financial Aid.

طراحی قالب وردپرس طراحی اپلیکیشن تور ترکیه ساخت اپلیکیشن آموزش وردپرس میز اداری صندلی مدیریتی صندلی اداری تبلیغات کلیکی میز تلویزیون پاراگلایدر آگهی رایگان آموزش وردپرس تور استانبول تور آنتالیا آموزش ووکامرس